About
Shuichi Sato, Ph.D., ATC
Associate professor, Interim Director, School of Kinesiology
Researcher at New Iberia Research Center (NIRC)
University of Louisiana at Lafayette
Office: Bourgeois Hall 129B, Email: s.sato@louisiana.edu
My research interest lies in the alternation of skeletal muscle size, physiology, and function due to aging, cancer, and disuse and effects of exercise training in addition to pharmacological/nutritional reagents using in vitro and in vivo models.
My research career began in the Analytical Chemistry lab at Sophia University, where I investigated the solvent-solute interaction between the organic solvent and organometallic complex using mathematical models. Since I moved to the US, my interest shifted toward biology/physiology in humans ending up working on research at the Integrative Muscle Biology lab of Dr. Carson in the Univ. of South Carolina. After getting a terminal degree at the institution, I had further research training at Dr. Kumar's lab in the University of Louisville, School of Medicine.
I played football (not soccer) in high school and college in my country. So football is still my favorite sport, and I like to watch football games in my spare time. But recently aging process teaches me how vital physical activity/exercise is, mandating me to step on a treadmill regularly. This experience further motivates me to study the role of exercise in fighting against common chronic diseases.
Lab capability
Bourgeois Hall
- Western blots
- - Mini-PROTEAN Tetra System (Bio-Rad)
- - Trans-Blot Turbo Transfer System (Bio-Rad)
- - Wet/Tank blotting system (Bio-Rad)
- - ChemiDoc Touch imaging system (Bio-Rad)
- RNA isolation and qRT-PCR
- - QIAcube (Qiagen)
- - MiniAmp thermal cycler (Applied Biosystems)
- - Mastercycler gradient thermal cycler(Eppendorf)
- - Rotor-Gene Q (Qiagen)
- Calorimetric assay and ELISA
- - Multiskan Sky (Thermofisher)
- - ELx808 (BioTek)
- Fluorometer/Spectrophotometer
- - Qubit 3.0 (Invitrogen)
- - Evolution 60S (Thermofisher)
- - Biophotometer (Eppendorf)
- - EZDrop 1000 Micro-volume Spectrophotometer (Blue-Ray Biotech)
- Agarose gel electrophoresis
- - Mini-Sub Cell GT gel electrophoresis system (Bio-Rad)
- - E-Gel Power Snap electrophoresis system and camera (Thermofisher)
- Histology
- - BA310 Light microscope with camera (Motic)
- Cell culture system
- - Biosafety cabinet (Baker, SG-400M)
- - Heracell CO2 incubators (Thermofisher)
- - Inverted microscope (Fisher Scientific)
- Mouse housing/exercise equipment
- - Mouse microisolator caging system (Ancare)
- - Mouse treadmill (Columbus Instruments)
- - In-vivo muscle stimulation system (Aurora Scientific)
- RNA isolation and qRT-PCR
- - Thermal cycler (Bio-Rad and Applied Biosystems)
- - StepOnePlus (Applied Biosystems)
- Flow cytometry and cell sorting
- - FacsCalibur Flow Cytometer (Becton Dickenson)
To be continued
New Iberia Research Center (NIRC)
To be continued
Education & Training
2015 - Post-Doc, Cell Biology, Univ. of Louisville School of Medicine
2012 - Ph.D., Applied Physiology, Univ. of South Carolina
2005 - MS, Exercise Science, Univ. of Southern Mississippi
2003 - BS, Athletic Training, Univ. of Southern Mississippi
1996 - MS, Chemistry, Sophia University
1994 - BS, Chemistry, Sophia University
Grants
Active
Advance Student Research Experience Grants (PI: Sato, 2023-2024)
Microvolume Spectrophotometer for Kinesiology Undergraduate Research
Advance Faculty Grant, Student Center for Research, Creativity and Scholarship (SCRCS, PI: Sato, 2023-2024)
Title:Wearable devices accuracy in measuring cardiac conditions during exercise
Louisiana BoRSF Departmental Enhancement Program (PI: Sato, 2023-2024)
Title: Upgrading Kinesiology Lab Equipment to Enhance Students’ Learning Outcomes
Completed
Louisiana Council on Excellence in Undergraduate Research (LaCOUER, PI: Sato, 2022-2023)
Title:The role of MST1 in Lewis Lung Carcinoma-induced myotube atrophy
Louisiana BoRSF Research Competitiveness Subprogram (PI: Sato, 2017-2021)
Title:The effect of Yes-associated protein YAP during the development of cancer cachexia
Yamada Research Fund (PI: Sato, 2016-2017)
Title: The effect of royal jelly on satellite cell activation in aged nonhuman primate in vitro
Research Interests
Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis
Kurosaka M, Ogura Y, Sato S, Kohda K, Funabashi T.
Skelet Muscle. 2021 May 29;11(1):14. doi: 10.1186/s13395-021-00271-8.
Keywords: Differentiation, Interleukin-4, Myoblast fusion, Myotube, Primary myoblast
Age-related decrease in muscle satellite cells is accompanied with diminished expression of early growth response 3 in mice
Ogura Y, Sato S, Kurosaka M, Kotani T, Fujiya H, Funabashi T.
Mol Biol Rep. 2020 Feb;47(2):977-986. doi: 10.1007/s11033-019-05189-5.
Keywords: Myogenesis, FACS, Stem cells, Sarcopenia, Regeneration
High-Frequency Stimulation on Skeletal Muscle Maintenance in Female Cachectic Mice.
Sato S, Gao S, Puppa MJ, Kostek MC, Wilson LB, Carson JA.
Med Sci Sports Exerc. 2019 Sep;51(9):1828-1837. doi: 10.1249/MSS.0000000000001991.
Keywords: Cachexia, mTOR, protein synthesis, resistance exercise
TAK1 regulates skeletal muscle mass and mitochondrial function.
Hindi SM, Sato S, Xiong G, Bohnert KR, Gibb AA, Gallot YS, McMillan JD, Hill BG, Uchida S, Kumar A.
JCI Insight. 2018 Feb 8;3(3). pii: 98441.
Keywords: Metabolism, Mouse models, Muscle Biology, Skeletal muscle
Inhibition of ER stress and unfolding protein response pathways causes skeletal muscle wasting during cancer cachexia
Bohnert KR, Gallot YS, Sato S, Xiong G, Hindi SM, and Kumar A.
FASEB J. 2016 Sep;30(9):3053-68. doi: 10.1096/fj.201600250RR.
Keywords: Cancer cachexia, ER stress, skeletal muscle, AKT, Unfolding protein response
Eccentric Contraction-Induced Myofiber Growth in Tumor-Bearing Mice
Hardee JP, Mangum J, Gao S, Sato S, Hetzler KL, Puppa MH, Fix D, and Carson JA.
J Appl Physiol (1985). 2016 Jan 1;120(1):29-37. doi: 10.1152/japplphysiol.00416.2015.
Keywords: cancer cachexia, eccentric contractions, high-frequency electrical stimulation, muscle inflammation, myofiber growth
TAK1 modulates satellite stem cell homeostasis and skeletal muscle repair
Ogura Y, Hindi SM, Sato S, Xiong G, Akira S, and Kumar A.
Nat Commun. 2015 Dec 9;6:10123. doi: 10.1038/ncomms10123
Keywords: transforming growth factor-b-activated kinase 1, TAK1, muscle regeneration, satellite cell
Sex differences in the relationship of IL-6 signalling to cancer cachexia progression
Hetzler KL, Hardee JP, Puppa MJ, Narsale AA, Sato S, Davis JM, and Carson JA.
Biochim Biophys Acta. 2015 May;1852(5):816-25. doi: 10.1016/j.bbadis.2014.12.015.
Keywords: Apc(Min/+), IL-6r, SOCS3, STAT3, Skeletal muscle.
Elevated levels of TWEAK in skeletal muscle promote visceral obesity, insulin resistance, and metabolic dysfunction
Sato S, Ogura Y, Tajrishi MM, and Kumar A.
FASEB J. 2015 Mar;29(3):988-1002. doi: 10.1096/fj.14-260703.
Keywords: AMPK, Fn14, GLUT4, PGC-1α, type 2 diabetes
Therapeutic potential of matrix metalloproteinases in Duchenne muscular dystrophy
Ogura Y, Tajrishi MM, Sato S, Hindi SM, Kumar A.
Front Cell Dev Biol. 2014 Apr 1;2:11. doi: 10.3389/fcell.2014.00011. eCollection 2014.
Keywords: NF-κB, TIMPs, fibrosis, inflammation, macrophages, matrix metalloproteinases, skeletal muscle
The TWEAK-Fn14 dyad is involved in age-associated pathological changes in skeletal muscle
Tajrishi MM, Sato S (equal contribution), Shin J, Zheng TS, Burkly LC, Kumar A.
Biochem Biophys Res Commun. 2014 Apr 18;446(4):1219-24. doi: 10.1016/j.bbrc.2014.03.084.
Keywords: Autophagy, Cytokines, NF-κB, Sarcopenia, Ubiquitin–proteasome system
TWEAK/Fn14 Signaling Axis Mediates Skeletal Muscle Atrophy and Metabolic Dysfunction
Sato S, Ogura Y, and Kumar A.
Front Immunol. 2014 Jan 27;5:18. doi: 10.3389/fimmu.2014.00018. eCollection 2014.
Keywords: Fn14, TWEAK, atrophy, oxidative phosphorylation, skeletal muscle
Distinct roles of TRAF6 at early and late stages of muscle pathology in the mdx model of Duchenne muscular dystrophy
Hindi SM, Sato S, Choi Y, and Kumar A
Hum Mol Genet. 2014 Mar 15;23(6):1492-505. doi: 10.1093/hmg/ddt536.
Keywords: TRAF6, Duchenne muscular dystrophy, nf-kb
TWEAK promotes exercise intolerance by decreasing skeletal muscle oxidative phosphorylation capacity
Sato S, Ogura Y, Mishra V, Shin J, Bhatnagar S, Hill BG, and Kumar A
Skelet Muscle. 2013 Jul 8;3(1):18. doi: 10.1186/2044-5040-3-18.
Keywords: Skeletal muscle, exercise tolerance, TWEAK, Fn14, PGC-1α, PPARδ
Muscle mTORC1 suppression by IL-6 during cancer cachexia: a role for AMPK
White JP, Puppa MJ, Gao S, Sato S, Welle SL, and Carson JA
Am J Physiol Endocrinol Metab. 2013 May 15;304(10):E1042-52. doi: 10.1152/ajpendo.00410.2012
Keywords: AMPK, IGF-I, IL-6, STAT, cachexia, colorectal cancer, exercise, inflammation, mTOR, muscle, protein synthesis, wasting
Development of an UPLC mass spectrometry method for measurement of myofibrillar protein synthesis: application to analysis of murine muscles during cancer cachexia.
Lima M, Sato S, Enos RT, Baynes JW, and Carson JA
J Appl Physiol (1985). 2013 Mar 15;114(6):824-8. doi: 10.1152/japplphysiol.01141.2012.
Keywords: cachexia, myofibrillar protein synthesis, soleus, plantaris, ultra-performance liquid chromatography mass spectrometry
Testosterone regulation of Akt/mTORC1/FoxO3a signaling in skeletal muscle
White JP, Gao S, Puppa MJ, Sato S, Welle SL, and Carson JA.
Mol Cell Endocrinol. 2013 Jan 30;365(2):174-86. doi: 10.1016/j.mce.2012.10.019.
Keywords: Muscle, Testosterone, FOXO3a, Akt, mTOR, AMPK
IL-6 regulation of muscle mitochondrial biogenesis and fission/fusion dynamics during the progression of cancer cachexia
White JP, Sato S, Puppa MJ, Baynes JW, Kostek, MC, Matesic LE and Carson JA.
Skeletal Muscle. 2012 Jul 6;2:14. doi: 10.1186/2044-5040-2-14.
Keywords: FIS1, PGC-1α, Exercise, IL-6r, MFN1, Cachexia, Mitochondria, Muscle, Autophagy
The Effect of Exercise on IL-6-induced Cachexia in the ApcMin/+ mouse
Puppa MJ, White JP, Velasquez KT, Baltgalvis KA, Sato S, Baynes JW, and Carson JA.
J Cachexia Sarcopenia Muscle. 2012 Jun;3(2):117-37. doi: 10.1007/s13539-011-0047-1.
Keywords: Inflammation, Colorectal cancer, Insulin resistance, Oxidative capacity
Gut barrier dysfunction in the ApcMin/+ mouse model of colon cancer cachexia
Puppa MJ, White JP, Sato S, Cairns M, Baynes JW, and Carson JA.
Biochimica et Biophysica Acta – Molecular Basis of Disease. Dec;1812(12):1601-6.
Keywords: Cachexia, Colorectal cancer, Endotoxin, Gut barrier dysfunction, Gut permeability, Inflammation
The regulation of skeletal muscle protein turnover during the progression of cancer cachexia in the ApcMin/+ mouse
White JP, Baynes JW, Welle SL, Kostek MC, Matesic LE, Sato S, and Carson JA.
PLoS ONE 6(9): e24650. doi:10.1371/journal.pone.0024650
Keywords: cachexia, protein synthesis, protein degradation, mTOR, FOXO, Ubiquitin–proteasome system
Skeletal muscle mass recovery from atrophy in IL-6 knockout mice
Washington TA, White JP, Davis JM, Wilson LB, Lowe LL, Sato S, and Carson JA.
Acta Physiol (Oxf). Acta Physiol (Oxf). 2011 Aug;202(4):657-69.
Keywords: inflammation, myogenesis, muscle regeneration, hypertrophy, IGF-1, mTOR, Stat3
Muscle oxidative capacity during IL-6 dependent cancer cachexia
White JP, Baltgalvis KA, Puppa MJ, Sato S, Baynes JW, and Carson JA.
Am J Physiol Regul Integr Comp Physiol; 2011 Feb;300(2):R201-11.
Keywords: mitochondria, wasting, mitofusin-2 protein, fission 1 protein
The effect of nandrolone decanoate administration on recovery from bupivacaine-induced muscle injury
White JP, Baltgalvis KA, Sato S, Wilson LB, and Carson JA
J Appl Physiol. 2009 Nov;107(5):1420-30.
Keywords: muscle regeneration, anabolic steroids, myotoxin
Overload-induced skeletal muscle extracellular matrix remodelling and myofibre growth in mice lacking IL-6.
White JP, Reecy JM, Washington TA, Sato S, Le ME, Davis JM, Wilson LB, and Carson JA
Acta Physiol (Oxf). 2009 Dec;197(4):321-32.
Keywords:
Announcement
Looking for lab members
Sato lab always looks for graduate/undergraduate students who are interested in skeletal muscle biology research. Please email Sato for details.
Requirements
A student seeking for a Master's degree is expected to enroll in Masters in Kinesiology. (FYI. No GRE score is required for the admission of this program).
If you are interested in Ph.D., admission to Doctorate in Biology Program is required while working in my lab.
News
Research Presentation
We presented a poster at the National ACSM Annual Meeting in June 2023. The title of our presentation was "Altered autophagic responses to mechanical overload in Lewis Lung Carcinoma-bearing mice".
Research Recognition
Our undergraduate student Bailey Nguyen won the Leaf Award conferred by the Student Center for Research, Creativity and Scholarship at UL Lafayette in March 2023. Congratulations, Bailey!
(Article->) Inaugural Leaf Awards recognize undergraduate student research, creativity, scholarship
Research Recognition
The American Kinesiology Association has recognized Emily Walker, an alumna of our lab, as the recipient of the 2022 AKA Masters Scholar Award.Congratulations, Emily!
Research Presentation
Our graduate student Alexis Milton provided her poster presentation at the National ACSM annual meeting in June 2022. The title of his presentation was "Cancer-induced changes in endoplasmic stress and autophagy responses after mechanical overload in ApcMin/+ mice".
Research Presentation
Our group provided a poster presentation at the Experimental Biology in April 2022. The title of our presentation was "The Effect of Glucose Administration on Yes-associated Protein (YAP) Activity in Healthy and Cachectic Mouse Muscles".
Research Presentation
Our graduate student Alexis Milton provided her poster presentation at the SEACSM annual meeting in February 2022. The title of his presentation was "Altered autophagy and endoplasmic reticulum stress response to mechanical overload in cachectic ApcMin/+ mice".
Manuscript Published
Editorial "Emerging Mechanisms for Skeletal Muscle Mass Regulation" has been published in Frontiers in Cell and Developmental Biology. doi: 10.3389/fcell.2021.764095.
Research Presentation
Our group provided a poster presentation at the 6th Cancer Cachexia Conference in August 2021. The title of his presentation was "Decreased Yes-associated protein response to eccentric muscle contractions in cachectic mice: A time-course study."
Research Presentation
Our group provided a poster presentation at the National ACSM meeting in June 2021. The title of his presentation was "Hippo Signaling Response To Eccentric Muscle Contractions In Cachectic Mouse Muscle."
Manuscript Published
The manuscript titled "Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis" has been published in Skeletal Muscle on May 29, 2021.
https://skeletalmusclejournal.biomedcentral.com/articles/10.1186/s13395-021-00271-8
Research Presentation
Our graduate student Richard won the 3rd place in the Master students' poster competition in SEACSM annual meeting in February 2021. The title of his presentation was "Muscle contraction by high-frequency electrical stimulation induces Hippo signaling effector YAP response in ApcMin/+ mice."
SEACSM Student Poster Competition Award Winners
— SEACSM (@seacsm) February 19, 2021
Master's Award Winners:
🥇Jillian Poles, University of North Carolina, Chapel Hill.
🥈Rachel Miller, Samford University.
🥉Richard Young, University of Louisiana - Lafayette.#SEACSM21 @ACSMNews
Research Presentation
Our graduate student Richard provided his poster presentation in Cancer Cachexia Conference in September 2020. The title of his presentation was "Increased sensitivity to systemic inflammation leads to elevated MMP-9 expression in mouse cachectic muscle".
Research Presentation
Our graduate student Richard provided his poster presentation in Experimental Biology in April 2020. The title of his presentation was "YAP Involves in Reduced Anabolic Response to Functional Overload in Lewis Lung Carcinoma-bearing Mice".
Guess who are there in the official SEACSM tweet?
Full room at early poster session today. #SEACSM20 pic.twitter.com/MDZa4qqxQG
— SEACSM (@seacsm) February 14, 2020
Research Presentation
Our graduate student Richard provided his poster presentation in Master's student poster competition at Southeast ACSM annual meeting in Jacksonville, FL in February 2020. The title of his presentation was "Reduced unfolded protein response is associated with anabolic resistance following mechanical overload in ApcMin/+ mice".
Research Presentation
Our undergraduate student Emily provided her presentation in the thematic poster session at Southeast ACSM annual meeting in Jacksonville, FL in February 2020. The title of her presentation was "Compromised anabolic response to mechanical overload leads to decreased muscle hypertrophy in tumor-implanted mice".
Research Presentation
Our undergraduate student Emily provided her poster presentation at LaCOEUR - Undergraduate Research Conference in Lafayette, LA in November 2019. The title of her presentation was "Impaired anabolic response leads to decreased muscle protein synthesis in colon cancer mice".
Publication
Our manuscript titled "Age-related decrease in muscle satellite cells is accompanied with diminished expression of early growth response 3 in mice" was accepted by Molecular Biology Report on 11/7/2019 and available online (here!).
Research Presentation
Our presentation led by Richard, a graduate student in Sato lab, was selected as a finalist for the student Master’s awards poster session for the SEACSM annual meeting in Jacksonville, FL in February 2020.
Research Presentation
Our undergraduate student Emily provided her poster presentation at Student Research Symposium at the UL Lafayette in May 2019. The title of her presentation was "Reduced mTOR response after 7-day mechanical overload in cachetic mice".
Research Presentation
Sato provided a poster presentation at Experimental Biology in Orlando, FL in April 2019. The title of presentation was "The Effect of Royal Jelly on Satellite Cell Activation in Aged Monkey Muscle Cells". The abstract can be found here on FASEB journal.
Publication
Our manuscript titled "High-frequency stimulation on skeletal muscle maintenance in female cachectic mice" was accepted by MSSE on 3/15/2019 and available online on 3/30/2019. See the following link (here!) for details.
Research Presentation
Our undergraduate student Kayla presented her poster titled "Impaired Compensatory Muscle Hypertrophy Following 7 days of Functional Overload in ApcMin/+ Mice" in 2019 Southeast ACSM annual meeting in February 2019. She was selected as a finalist for the student undergraduate awards poster session.
Research Presentation
Sato presented his poster at 11th International Conference on Cachexia, Sarcopenia, and Muscle wasting in Maastricht, Netherland in December 2018. The title of his poster was Potential involvement of Yes-Associated Protein in anabolic resistance during mechanical loading in cachectic mice.
Honors
Sato has been appointed to the Joan D. and Alexander S. Haig/BORSF Professorship in Education (2018-2021)
Research Presentation
Our undergraduate students Emily and Taylor presented their poster at Student Research Symposium at the UL Lafayette in April 2018. The title of their presentation was "Genotyping ApcMin/+ Mice with a standard PCR"
Publication
A new manuscript has been published in JCI insight on 2/8/2018. https://insight.jci.org/articles/view/98441
Funding
Sato received Travel Grants for Emerging Faculty (TGEF) from Louisiana Experimental Program to Stimulate Competitive Research (EPSCoR) in September 2017.
Research Presentation
Undergraduate students Janesha and Ben presented their poster at Experimental Biology 2017 in Chicago. The title of the presentation was "The Role of Hippo Signaling Pathway on Muscle Wasting in ApcMin/+ Mice: A Pilot Study".